A Simple Enzymatic Method for Quantitation of 2 '-Fucosyllactose

作者：Seydametova Emine; Shin Jonghyeok; Yu Jiwon; Kweon Dae-Hyuk^*

来源：Journal of Microbiology and Biotechnology, 2018, 28(7): 1141-1146.

DOI：10.4014/jmb.1803.03003

• 2 '-Fucosyllactose
• quantitation
• alpha-(1-2,3,4,6)-L-fucosidase
• L-fucose
• L-fucose dehydrogenase

2'-Fucosyllactose (2'-FL) is one of the most important human milk oligosaccharides and has several health benefits for infants. The levels of 2'-FL in breast milk or samples from other sources can be quantified by high-performance liquid chromatography. However, this method cannot be used for simultaneous detection of the target compound in numerous samples. Here, we developed a simple method for quantifying 2'-FL in a microplate format. The method involves two steps: (i) release of L-fucose from 2'-FL by alpha-(1-2,3,4,6)-L-fucosidase and (ii) measurement of NADPH formed during the oxidation of L-fucose by L-fucose dehydrogenase. This method enables measurement of up to 5 g/1 2'-FL in 50 min using a 96-well microplate. The efficiency and simplicity of the proposed method make it suitable for the analyses of a large number of samples simultaneously.

• 出版日期2018-7