An orthopoxvirus-based vaccine reduces virus excretion after MERS-CoV infection in dromedary camels

作者：Haagmans Bart L^*; van den Brand Judith M A; Raj V Stalin; Volz Asisa; Wohlsein Peter; Smits Saskia L; Schipper Debby; Bestebroer Theo M; Okba Nisreen; Fux Robert; Bensaid Albert; Foz David Solanes; Kuiken Thijs; Baumgaertner Wolfgang; Segales Joaquim; Sutter Gerd; Osterhaus Albert D M E

来源：Science, 2016, 351(6268): 77-81.

DOI：10.1126/science.aad1283

Middle East respiratory syndrome coronavirus (MERS-CoV) infections have led to an ongoing outbreak in humans, which was fueled by multiple zoonotic MERS-CoV introductions from dromedary camels. In addition to the implementation of hygiene measures to limit further camel-to-human and human-to-human transmissions, vaccine-mediated reduction of MERS-CoV spread from the animal reservoir may be envisaged. Here we show that a modified vaccinia virus Ankara (MVA) vaccine expressing the MERS-CoV spike protein confers mucosal immunity in dromedary camels. Compared with results for control animals, we observed a significant reduction of excreted infectious virus and viral RNA transcripts in vaccinated animals upon MERS-CoV challenge. Protection correlated with the presence of serum neutralizing antibodies to MERS-CoV. Induction of MVA-specific antibodies that cross-neutralize camelpox virus would also provide protection against camelpox.

• 出版日期2016-1-1