科研之友
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摘要
The intraneuronal chloride concentration ([Cl-](i)) is paramount for determining the polarity of signaling at GABA(A) synapses in the central nervous system. Sectioning hippocampal brain slices increases [Cl-](i) in the superficial layers. It is not known whether cutting trauma also increases [Cl-](i) in the neocortex and thalamus, and whether the effects of trauma change during development. We used Cl- imaging to study the [Cl-](i) vs. the distance from the cut surface in acute thalamocortical slices from mice at developmental ages ranging from postnatal day 5 (P5) to P20. We demonstrate: 1) [Cl-](i) is higher in the most superficial areas in both neocortical and thalamic brain slices at all ages tested and, 2) there is a developmental decrease in [Cl-](i) that is independent of acute trauma caused by brain slicing. We conclude that [Cl-](i) has a developmental progression during P5-20 in both the neocortex and thalamus. However, in both brain regions and during development the neurons closest to the slicing trauma have an elevated [Cl-](i) .
- 出版日期2016-6-23
