Activity and stability of horseradish peroxidase in hydrophilic room temperature ionic liquid and its application in non-aqueous biosensing

作者：Wang Sheng Fu; Chen Ting; Zhang Zhi Ling; Pang Dai Wen^*

来源：Electrochemistry Communications, 2007, 9(6): 1337-1342.

DOI：10.1016/j.elecom.2007.01.042

• room temperature ionic liquid
• horseradish peroxidase
• activity and stability
• 1-butyl-3-methylimidazolium tetrafluroborate
• hydrogen peroxide

The activity and stability of horseradish peroxidase (HRP) were investigated in a hydrophilic room temperature ionic liquid 1-butyl-3methylimidazolium. tetrafluroborate ([bmim][BF4]) by electrochemical methods. Although no detectable activity exhibited in anhydrous [bmim][BF4], HRP was active in the presence of a small amount of water (4.53%, v/v). And its activity can be improved by immobilization in agarose hydrogel. The immobilized HRP possesses excellent activity at 65 degrees C. It remained 80.2% of its initial activity after being immersed for 10.5 h in an aqueous mixture of [bmim][BF4] with some hydrogen peroxide (H2O2) under room temperature, implying extremely high stability. Moreover, the immobilized HRP was found to be very sensitive and stable in H2O-containing [bmim][BF4] for the detection of H2O2, with a wide linear range of 6. 10 x 10(-7) to 1.32 x 10(-4) Mol l(-1) and low detection limit of 1.0 x 10(-7) Mol l(-1).

• 出版日期2007-6
• 单位湖北大学; 武汉大学