HiChIP: efficient and sensitive analysis of protein-directed genome architecture

作者：Mumbach Maxwell R; Rubin Adam J; Flynn Ryan A; Dai Chao; Khavari Paul A; Greenleaf William J; Chang Howard Y^*

来源：Nature Methods, 2016, 13(11): 919-+.

DOI：10.1038/NMTH.3999

Genome conformation is central to gene control but challenging to interrogate. Here we present HiChIP, a protein-centric chromatin conformation method. HiChIP improves the yield of conformation-informative reads by over 10-fold and lowers the input requirement over 100-fold relative to that of ChIA-PET. HiChIP of cohesin reveals multiscale genome architecture with greater signal-to-background ratios than those of in situ Hi-C.

• 出版日期2016-11