Antioxidative capacity in the fat body of Bombyx mori is increased following oral administration of 4-methylumbelliferone

作者：Fang Yan; Wang Hua; Zhu Wenjuan; Wang Lu; Liu Hengjiang; He Yue; Xu Xu; Yin Weimin; Sima Yanghu; Xu Shiqing^*

来源：Comparative Biochemistry and Physiology C-Toxicology & Pharmacology, 2014, 159: 31-37.

DOI：10.1016/j.cbpc.2013.09.003

• 4-Methylumbelliferone
• Reactive oxygen species
• Anti-oxidant
• Oxidative stress response
• Bombyx mori
• Fat body

Plant sources of umbelliferones have tumor-inhibitory effects at the cellular level. However, their physiological functions in animals are largely unresolved. In this study, we provide evidence to show that 4-methylumbelliferone (4-MU) participates in the regulation of antioxidative capacity in the fat body of Bombyx mod, a tissue similar to mammalian liver in this model invertebrate. Larvae (3rd day of the 5th instar) were orally exposed to 4 mM 4-MU, an umbelliferone, which swiftly induced the generation of a large number of ROS (e.g. H2O2 increased 6 to 8-fold), and 4-MU was detected in the fat body 8 mm after administration. In addition, the activities of CAT and GPx were up-regulated 4 to 11-fold and 2 to 16-fold, respectively, and were helpful in defending fat body cells against oxidative injury in combination with NADPH. Furthermore, significant increases in the contents of T-AOC (up to approx. 2-fold), antioxidants of ASAFR (by 2 to 4-fold) and GSH were detected.

• 出版日期2014-1
• 单位苏州大学