Assessing mitochondrial morphology and dynamics using fluorescence wide-field microscopy and 3D image processing

作者：Song Wenjun; Bossy Blaise; Martin Ola J; Hicks Andrew; Lubitz Sarah; Knott Andrew B; Bossy Wetzel Ella^*

来源：Methods, 2008, 46(4): 295-303.

DOI：10.1016/j.ymeth.2008.10.003

• Mitochondrial fission
• Mitochondrial fusion
• Time-lapse imaging
• Fluorescence wide-field microscopy
• Motor neurons
• Astrocytes
• Mitochondrial length
• Mitochondrial number

Mitochondrial morphology and length change during fission and fusion and mitochondrial movement varies dependent upon the cell type and the physiological conditions. Here, we describe fundamental wide-field fluorescence microcopy and 3D imaging techniques to assess mitochondrial shape, number and length in various cell types including cancer cell lines, motor neurons and astrocytes. Furthermore, we illustrate how to assess mitochondrial fission and fusion events by 3D time-lapse imaging and to calculate mitochondrial length and numbers as a function of time. These imaging methods provide useful tools to investigate mitochondrial dynamics in health, aging and disease.

• 出版日期2008-12
• 单位河北医科大学