Molecular phylogeny of the marine sand-dwelling dinoflagellate Herdmania litoralis and an emended description of the closely related planktonic genus Archaeperidinium Jorgensen

作者：Yamaguchi Aika^*; Hoppenrath Mona; Pospelova Vera; Horiguchi Takeo; Leander Brian S

来源：European Journal of Phycology, 2011, 46(2): 98-112.

DOI：10.1080/09670262.2011.564517

• Archaeperidinium
• Herdmania litoralis
• heterotrophic dinoflagellates
• intraspecific variability
• LSU rDNA
• Protoperidiniaceae
• Protoperidinium minutum
• single-cell PCR
• SSU rDNA
• Thecadinium dragescoi

Herdmania litoralis is a heterotrophic, sand-dwelling dinoflagellate with morphological characters that do not provide clear evidence for its systematic position in any existing family of dinoflagellates. Protoperidinium minutum is a heterotrophic, planktonic species that has a typical tabulation for the genus Protoperidinium. In order to infer the phylogenetic positions of these two species more confidently, we characterized the thecal plate patterns and determined small-subunit and large-subunit ribosomal DNA sequences (SSU rDNA and LSU rDNA, respectively) from both species. Intraindividual and intraspecific diversity of SSU and LSU rDNA data were characterized in H. litoralis using a combination of single-cell PCR approaches and analyses of PCR clones derived from multi-cell DNA extractions. The results of the molecular phylogenetic analyses demonstrated a novel, well-supported clade comprising both sand-dwelling species (H. litoralis and Thecadinium dragescoi) and planktonic species (P. minutum). Because the establishment of this clade also demonstrated that P. minutum is not a member of Protoperidinium, we reinstated and emended the genus Archaeperidinium Jorgensen 1912.

• 出版日期2011