A yeast co-culture-based biosensor for determination of waste water contamination levels

作者：Yudina N Yu; Arlyapov V A; Chepurnova M A; Alferov S V; Reshetilov A N^*

来源：Enzyme and Microbial Technology, 2015, 78: 46-53.

DOI：10.1016/j.enzmictec.2015.06.008

• Co-culture
• Bacteria
• Yeasts
• Biochemical oxygen demand (BOD)
• Biosensor analyzer
• Growth curves

Artificial microbial co-cultures were formed to develop the receptor element of a biosensor for assessment of biological oxygen demand (BOD). The co-cultures possessed broad substrate specificities and enabled assays of water and fermentation products within a broad BOD range (2.4-80 mg/dm(3)) with a high correlation to the standard method (R=0.9988). The use of the co-cultures of the yeasts Pichia angusta, Arxula adeninivorans and Debaryomyces hansenii immobilized in N-vinylpyrrolidone-modified poly(vinyl alcohol) enabled developing a BOD biosensor possessing the characteristics not inferior to those in the known biosensors. The results are indicative of a potential of using these co-cultures as the receptor element base in prototype models of instruments for broad application.

• 出版日期2015-10