Antibacterial properties of amino acid functionalized silver nanoparticles decorated on graphene oxide sheets

作者：Chandraker Kumudini; Nagwanshi Rekha; Jadhav S K; Ghosh Kallol K; Satnami Manmohan L^*

来源：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy , 2017, 181: 47-54.

DOI：10.1016/j.saa.2017.03.032

• Graphene oxide
• L-cys-AgNPs
• GO-L-cys-Ag nanocomposite
• Antibacterial activity
• SEM

Graphene oxide (GO) sheets decorated with amino acid L-cysteine (L-cys) functionalized silver nanoparticles (GO-L-cys-Ag) was synthesized by AgNO3, trisodium citrate, and NaBH4. GO-L-cys-Ag nanocomposite was characterized by transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectra, ultraviolet-visible (UV-vis) absorption spectra, which demonstrated that a diameter of L-cys-AgNPs compactly deposited on GO. Antibacterial activity tests of GO-L-cys-Ag nanocomposite were carried out using Escherichia coli MTCC 1687 and Staphylococcus aureus MTCC 3160 as model strains of Gram-negative and Gram-positive bacteria, respectively. The effect of bactericide dosage on antibacterial activity of GO-L-cys-Ag nanocomposite was examined by plate count, well diffusion and broth dilution methods. Morphological observation of bacterial cells by scanning electron microscope (SEM) showed that GO-L-cys-Ag nanocomposite was more destructive to cell membrane of Escherichia coli than that of Staphylococcus aureus. The above technique establish that the bactericidal property of GO-L-cys-Ag nanocomposite with wide range of applications in biomedical science.

• 出版日期2017-6-15