Antinuclear antibodies as ancillary markers in primary biliary cirrhosis

作者：Granito Alessandro^*; Muratori Paolo; Quarneti Chiara; Pappas Georgios; Cicola Ronny; Muratori Luigi

来源：Expert Review of Molecular Diagnostics, 2012, 12(1): 65-74.

DOI：10.1586/ERM.11.82

• antinuclear antibodies
• gp210
• multiple nuclear dots
• nuclear pore complex
• prognosis
• rim-like/membranous
• sp100
• surrogate markers

Antimitochondrial antibodies are the serological hallmark of primary biliary cirrhosis (PBC). Besides antimitochondrial antibodies, the autoantibody profile of PBC includes antinuclear antibodies (ANA) which are detectable by indirect immunofluorescence in up to 50% of PBC patients. Two immunofluorescence patterns are considered %26apos;PBC-specific%26apos;: the multiple nuclear dots and rim-like/membranous patterns. The target antigens of the multiple nuclear dots pattern have been identified as Sp100 and promyelocytic leukemia protein, whereas the rim-like/membranous pattern is given by autoantibodies recognizing multiple proteins such as gp210, nucleoporin p62 and the lamin B receptor. Other ANA, especially those already known in the rheumatological setting, such as anticentromere, anti-SSA/Ro and anti-dsDNA antibodies, can be frequently found in PBC, often coexisting in the same patient. In this article, we will report on recent progress in the antigenic characterization of ANA in PBC, their detection with both traditional assays and Western blot/ELISA with molecularly defined nuclear antigens, and we will discuss their clinical significance.

• 出版日期2012-1