MicroRNA signature analysis in colorectal cancer: identification of expression profiles in stage II tumors associated with aggressive disease

作者：Chang Kah Hoong; Miller Nicola^*; Kheirelseid Elrasheid A H; Lemetre Christophe; Ball Graham R; Smith Myles J; Regan Mark; McAnena Oliver J; Kerin Michael J

来源：International Journal of Colorectal Disease, Clinical and Molecular Gastroenterology and Surgery, 2011, 26(11): 1415-1422.

DOI：10.1007/s00384-011-1279-4

• Colorectal cancer
• MicroRNA
• Expression signature
• Artificial neural networks

Purpose Colorectal cancer (CRC) is a clinically diverse disease whose molecular etiology remains poorly understood. The purpose of this study was to identify miRNA expression patterns predictive of CRC tumor status and to investigate associations between microRNA (miRNA) expression and clinicopathological parameters.
Methods Expression profiling of 380 miRNAs was performed on 20 paired stage II tumor and normal tissues. Artificial neural network (ANN) analysis was applied to identify miRNAs predictive of tumor status. The validation of specific miRNAs was performed on 102 tissue specimens of varying stages.
Results Thirty-three miRNAs were identified as differentially expressed in tumor versus normal tissues. ANN analysis identified three miRNAs (miR-139-5p, miR-31, and miR-1792 cluster) predictive of tumor status in stage II disease. Elevated expression of miR-31 (p= 0.004) and miR-139-5p (p< 0.001) and reduced expression of miR-143 (p= 0.016) were associated with aggressive mucinous phenotype. Increased expression of miR-10b was also associated with mucinous tumors (p= 0.004). Furthermore, progressively increasing levels of miR-10b expression were observed from T1 to T4 lesions and from stage I to IV disease.
Conclusion Association of specific miRNAs with clinicopathological features indicates their biological relevance and highlights the power of ANN to reliably predict clinically relevant miRNA biomarkers, which it is hoped will better stratify patients to guide adjuvant therapy.

• 出版日期2011-11