Microarray Analysis of Oligosaccharide-Mediated Multivalent Carbohydrate-Protein Interactions and Their Heterogeneity

作者：Gade Madhuri; Alex Catherine; Ben Arye Shani Leviatan; Monteiro Joao T; Yehuda Sharon; Lepenies Bernd^*; Padler Karavani Vered^*; Kikkeri Raghavendra^*

来源：ChemBioChem, 2018, 19(11): 1170-1177.

DOI：10.1002/cbic.201800037

• C-type lectin
• heterogeneity
• mannose
• microarray
• oligosaccharides

C-type lectin , heterogeneity , mannose , microarray , oligosaccharidesCarbohydrate-protein interactions (CPIs) are involved in a wide range of biological phenomena. Hence, the characterization and presentation of carbohydrate epitopes that closely mimic the natural environment is one of the long-term goals of glycosciences. Inspired by the multivalency, heterogeneity and nature of carbohydrate ligand-mediated interactions, we constructed a combinatorial library of mannose and galactose homo-and hetero-glycodendrons to study CPIs. Microarray analysis of these glycodendrons with a wide range of biologically important plant and animal lectins revealed that oligosaccharide structures and heterogeneity interact with each other to alter binding preferences.

• 出版日期2018-6-4