Association between allelic variation due to short tandem repeats in tRNA gene of Entamoeba histolytica and clinical phenotypes of amoebiasis

作者：Jaiswal Virendra; Ghoshal Ujjala; Mittal Balraj; Dhole Tapan N; Ghoshal Uday C

来源：Acta Tropica, 2014, 133: 1-7.

DOI：10.1016/j.actatropica.2014.01.009

• Entamoeba histolytica
• Amoebiasis
• tRNA-linked STRs polymorphism
• Allele

Genotypes of Entamoeba histolytica (E. histolytica) may contribute clinical phenotypes of amoebiasis such as amoebic liver abscess (ALA), dysentery and asymptomatic cyst passers state. Hence, we evaluated allelic variation due to short tandem repeats (STRs) in tRNA gene of E. histolytica and clinical phenotypes of amoebiasis. Asymptomatic cyst passers (n = 24), patients with dysentery (n = 56) and ALA (n = 107) were included. Extracted DNA from stool (dysentery, asymptomatic cyst passers) and liver aspirate was amplified using 6 E. histolytica specific tRNA-linked STRs (D-A, A-L, N-K2, R-R, S-Q and S-TGA-D) primers. PCR products were subjected to sequencing. Association between allelic variation and clinical phenotypes was analyzed. A total of 9 allelic variations were found. in D-A, 8 in A-L, 4 in N-K2, 5 in R-R, 10 in STD and 7 in S-Q loci. A significant association was found between allelic variants and clinical phenotypes of amoebiasis. This study reveals that allelic variation due to short tandem repeats (STRs) in tRNA gene of E. histolytica is associated different clinical outcome of amoebiasis.

• 单位
  河北医科大学