The novel protein C9orf116 promotes rat liver cell line BRL-3A proliferation

作者：Zhang, Chunyan; Chang, Cuifang; Zhao, Weiming; Gao, Hang; Wang, Qiwen; Li, Deming; Zhang, Fuchun; Zhang, Shifu; Xu, Cunshuan^*

来源：PLos One, 2017, 12(7): e0180607.

DOI：10.1371/journal.pone.0180607

• IN-VIVO
• HEPATOCYTE PROLIFERATION
• DIFFERENTIAL REGULATION
• LENTIVIRAL VECTORS
• GENE-EXPRESSION
• GROWTH-HORMONE
• CYCLIN B1
• C-JUN
• REGENERATION
• PHASE

Our previous study has proved that the chromosome 9 open reading frame 116 (C9orf116) (NM_001106564.1) was significantly up-regulated in the proliferation phase of liver regeneration. To study its possible physiological function, we analyzed the effect of C9orf116 on BRL-3A cells via over-expression and interference technique. MTT results showed that the cell viability of the interference group was significantly lower than the control group at 48h after transfection (P<0.05), whereas it was significantly higher in the over-expression group (P<0.05). The flow cytometry results showed that C9orf116 knockdown or over-expression had little effect on BRL-3A cell apoptosis. However, the number of cells in division phase (G2/M) was significantly reduced in the interference group (P<0.05), but significantly increased in the over-expression group (P<0.01). Furthermore, the expressions of cell proliferation-related genes CCNA2, CCND1 and MYC both at mRNA and protein levels were down-regulated in the interference group and up-regulated in the over-expression group. Therefore, we concluded that C9orf116 may promote cell proliferation by modulating cell cycle transition and the expression of key genes CCNA2, CCND1 and MYC in BRL-3A cells.

• 出版日期2017-7-27
• 单位新疆大学; 河南师范大学