An SECM study on the influence of cationic, membrane-active peptides on a gold-supported self-assembled monolayer

作者：Abdelhamid Muhammad E; Piantavigna Stefania; Bond Alan M; Graham Bim; Spiccia Leone; Martin Lisandra L^*; O'Mullane Anthony P

来源：Electrochemistry Communications, 2015, 51: 11-14.

DOI：10.1016/j.elecom.2014.11.018

• SECM
• Self-assembled monolayers
• Electron transfer
• Peptides

The influence of the membrane active peptides, Tat(44-57) (activator in HIV-1) and melittin (active content of bee venom), on self-assembled monolayers of 6-mercaptohexanoic acid (MHA) on gold electrodes has been studied with scanning electrochemical microscopy (SECM). It was found that MHA, when deprotonated at physiological pH, significantly affected the relative rates of electron transfer between the [Fe(CN)(6)](4-) solution based mediator and the underlying gold electrode, predominantly by the electrostatic interaction between the mediator and MHA. Upon the introduction of Tat(44-57) or melittin to the electrolyte, the relative rate of electron transfer through the MHA layer could be increased or decreased depending on the mediator used. However, in all cases it was found that these peptides have the ability to be incorporated into synthetic SAMs, which has implications for future electrochemical studies carried out using cell mimicking membranes immobilised on such layers.

• 出版日期2015-2