A Hyperandrogenic Mouse Model to Study Polycystic Ovary Syndrome

作者：Xue, Ping; Wang, Zhiqiang; Fu, Xiaomin; Vvang, Junjiang; Punchhi, Gopika; Wolfe, Andrew; Wu, Sheng^*

来源：Jove-Journal of Visualized Experiments, 2018, (140): e58379.

DOI：10.3791/58379

• Developmental Biology
• Issue 140
• Polycystic ovary syndrome
• Dihydrotestosterone (DHT)
• Estrous cyclicity
• Androgen
• Puberty
• female offspring

Hyperandrogenemia plays a critical role in reproductive and metabolic function in females and is the hallmark of polycystic ovary syndrome. Developing a lean PCOS-like mouse model that mimics women with PCOS is clinically meaningful. In this protocol, we describe such a model. By inserting a 4 mm length of DHT (dihydrotestosterone) crystal powder pellet (total length of pellet is 8 mm), and replacing it monthly, we are able to produce a PCOS-like mouse model with serum DHT levels 2 fold higher than mice not implanted with DHT (no-DHT). We observed reproductive and metabolic dysfunction without changing body weight and body composition. While exhibiting a high degree of infertility, a small subset of these PCOS-like female mice can get pregnant and their offspring show delayed puberty and increased testosterone as adults. This PCOS-like lean mouse model is a useful tool to study the pathophysiology of PCOS and the offspring from these PCOS-like dams.

• 出版日期2018-10
• 单位南方医科大学; 中国人民解放军陆军总医院