Dynamic contrast-enhanced-MRI of tumor hypoxia

作者：Egeland Tormod A M; Gulliksrud Kristine; Gaustad Jon Vidar; Mathiesen Berit; Rofstad Einar K^*

来源：Magnetic Resonance in Medicine, 2012, 67(2): 519-530.

DOI：10.1002/mrm.23014

• DCE-MRI
• pharmacokinetic analysis
• Ktrans and ve
• tumor hypoxia

Patients with highly hypoxic primary tumors show increased frequency of locoregional treatment failure and poor survival rates and may benefit from particularly aggressive treatment. The potential of gadolinium diethylene-triamine penta-acetic acid-based dynamic contrast-enhanced-MRI in assessing tumor hypoxia was investigated in this preclinical study. Xenografted tumors of eight human melanoma lines were subjected to dynamic contrast-enhanced-MRI and measurement of the fraction of radiobiologically hypoxic cells and the fraction of pimonidazole-positive hypoxic cells. Tumor images of Ktrans (the volume transfer constant of gadolinium diethylene-triamine penta-acetic acid) and ve (the fractional distribution volume of gadolinium diethylene-triamine penta-acetic acid) were produced by pharmacokinetic analysis of the dynamic contrast-enhanced-MRI data, and Ktrans and ve frequency distributions of the non-necrotic tumor tissue were established and related to the extent of hypoxia. Tumors showing high Ktrans values and high ve values had low fractions of hypoxic cells, whereas tumors showing both low Ktrans values and low ve values had high hypoxic fractions. Ktrans differentiated better between tumors with low and high hypoxic fractions than did ve. This study supports the current attempts to establish dynamic contrast-enhanced-MRI as a method for assessing the extent of hypoxia in human tumors, and it provides guidelines for the clinical development of valid assays. Magn Reson Med, 2012.

• 出版日期2012-2