A comparative study: Metal nanoparticles as fluorescent sensors for biomolecules and their biomedical application

作者：Makwana Bharat A^*; Darjee Savan; Jain Vinod K; Kongor Anita; Sindhav Gaurang; Rao Mandava V

来源：Sensors and Actuators B: Chemical , 2017, 246: 686-695.

DOI：10.1016/j.snb.2017.02.054

• Calix[4]resorcinarene
• Fluorescent
• Gold nanoparticles
• Silver nanoparticles

In the present study, the interaction of the calix[4]resorcinarene polyhydrazide (CPH) capped nanoparticles (CPH-AuNps) and (CPH-AgNps) with different amino acids like arginine, aspartic acid, glutamic acid, glycine, leucine, methionine, histidine, tryptophan and cysteine has been studied by using UV-vis and fluorescence spectroscopy. CPH-AuNps and CPH-AgNps were found to be the selective and sensitive fluorescent probe for L-DOPA and histidine, respectively. The minimum detectable limit for both L-DOPA and histidine was found at 10 nM by a facile way of fluorescence quenching i.e. by a turn off mechanism. CPH-AuNps and CPH-AgNps have also been studied for their antioxidant activity and interaction with DNA by UV-vis spectrophotometry. Furthermore, electrophoresis technique confirmed the interaction of ethidium bromide (EtBr) intercalated CT-DNA and S-DNA with CPH-AuNps and CPH-AgNps, respectively. The simplicity, sensitivity and specificity for the detection of amino acids by the CPH-AuNps and CPH-AgNps, indicates that the synthesized nanoparticles can be used as nano biosensor, with a potential prospect in the biomedical analysis.

• 出版日期2017-7