Alveolar Epithelial Cell Injury Due to Zinc Oxide Nanoparticle Exposure

作者：Kim Yong Ho; Fazlollahi Farnoosh; Kennedy Ian M; Yacobi Nazanin R; Hamm Alvarez Sarah F; Borok Zea; Kim Kwang Jin; Crandall Edward D^*

来源：American Journal of Respiratory and Critical Care Medicine, 2010, 182(11): 1398-1409.

DOI：10.1164/rccm.201002-0185OC

• epithelial monolayers
• zinc toxicity
• reactive oxygen species
• mitochondrial damage
• plasma membrane integrity

Rationale: Although inhalation of zinc oxide (ZnO) nanoparticles (NPs) is known to cause systemic disease (i.e., metal fume fever), little is known about mechanisms underlying injury to alveolar epithelium.
Objectives: Investigate ZnO NP-induced injury to alveolar epithelium by exposing primary cultured rat alveolar epithelial cell monolayers (RAECMs) to ZnO NPs.
Methods: RAECMs were exposed apically to ZnO NPs or, in some experiments, to culture fluid containing ZnCl(2) or free Zn released from ZnO NPs. Transepithelial electrical resistance (R(T)) and equivalent short-circuit current (I(EQ)) were assessed as functions of concentration and time. Morphologic changes, lactate dehydrogenase release, cell membrane integrity, intracellular reactive oxygen species (ROS), and mitochondrial activity were measured.
Measurements and Main Results: Apical exposure to 176 mu g/ml ZnO NPs decreased R(T) and I(EQ) of RAECMs by 100% over 24 hours, whereas exposure to 11 mu g/ml ZnO NPs had little effect. Changes in R(T) and I(EQ) caused by 176 mu g/ml ZnO NPs were irreversible. ZnO NP effects on R(T) yielded half-maximal concentrations of approximately 20 mu g/ml. Apical exposure for 24 hours to 176 mu g/ml ZnO NPs induced decreases in mitochondrial activity and increases in lactate dehydrogenase release, permeability to fluorescein sulfonic acid, increased intracellular ROS, and translocation of ZnO NPs from apical to basolateral fluid (most likely across injured cells and/or damaged paracellular pathways).
Conclusions: ZnO NPs cause severe injury to RAECMs in a dose-and time-dependent manner, mediated, at least in part, by free Zn released from ZnO NPs, mitochondrial dysfunction, and increased intracellular ROS.

• 出版日期2010-12-1