A rapid, instrument-free, sample-to-result nucleic acid amplification test

作者：Lafleur Lisa K; Bishop Joshua D; Heiniger Erin K; Gallagher Ryan P; Wheeler Maxwell D; Kauffman Peter; Zhang Xiaohong; Kline Enos C; Buser Joshua R; Kumar Sujatha; Byrnes Samantha A; Vermeulen Nicolaas M J; Scarr Noah K; Belousov Yevgeniy; Mahoney Walt; Toley Bhushan J; Ladd Paula D; Lutz Barry R; Yager Paul

来源：Lab on a Chip, 2016, 16(19): 3777-3787.

DOI：10.1039/c6lc00677a

The prototype demonstrated here is the first fully integrated sample-to-result diagnostic platform for performing nucleic acid amplification tests that requires no permanent instrument or manual sample processing. The multiplexable autonomous disposable nucleic acid amplification test (MAD NAAT) is based on two-dimensional paper networks, which enable sensitive chemical detection normally reserved for laboratories to be carried out anywhere by untrained users. All reagents are stored dry in the disposable test device and are rehydrated by stored buffer. The paper network is physically multiplexed to allow independent isothermal amplification of multiple targets; each amplification reaction is also chemically multiplexed with an internal amplification control. The total test time is less than one hour. The MAD NAAT prototype was used to characterize a set of human nasal swab specimens pre-screened for methicillin-resistant Staphylococcus aureus (MRSA) bacteria. With qPCR as the quantitative reference method, the lowest input copy number in the range where the MAD NAAT prototype consistently detected MRSA in these specimens was similar to 5 x 10(3) genomic copies (similar to 600 genomic copies per biplexed amplification reaction).

• 出版日期2016