Association of polymorphisms of the serotonergic system with smoking initiation in Caucasians

作者：Iordanidou Maria; Tavridou Anna; Petridis Ioannis; Kyroglou Soultana; Kaklamanis Loukas; Christakidis Dimitrios; Manolopoulos Vangelis G^*

来源：Drug and Alcohol Dependence, 2010, 108(1-2): 70-76.

DOI：10.1016/j.drugalcdep.2009.11.015

• Gender
• 5-HT(2C) receptor gene polymorphisms
• 5-HTTLPR polymorphism
• Smoking
• Smoking initiation
• Caucasians

Background: The serotonergic system may be implicated in susceptibility to nicotine dependence as nicotine increases 5-hydroxytryptamine (5-HT) release in brain and symptoms of nicotine withdrawal may be modulated by diminished serotonergic neurotransmission. We examined the association of polymorphisms of genes involved in release and receptor function of 5-HT with cigarette smoking initiation in subjects of Caucasian origin.
Methods: 5-HTTLPR polymorphism Of the 5-HT transporter gene and -759C/T(rs3813929) and -697G/C (rs518147) polymorphisms of the 5-HT(2C) receptor gene were analyzed in 172 smoking initiators and 254 non-initiators, using PCR-RFLP method. Smoking behavior was assessed with a questionnaire about tobacco use.
Results: We found no differences in the frequency of the 5-HTTLPR genotypes between smoking initiators and non-initiators. However, the frequency of 5-HT(2C)-759T allele was significantly higher in non-initiators than smoking initiators (29.5% vs 16.3%, p = 0.002) and the same was true for 5-HT(2C)-697C allele carriers (48.8% vs 34.9%. p=0.004). Sex-dependent analysis revealed that these increased frequencies of -759T and -697C allele carriers were present only in males. No association was observed between any quantitative measures of smoking and these three polymorphisms.
Conclusions: 5-HTTLPR polymorphism was not associated with smoking initiation in either male or female subjects. However, significant association was found between 5-HT(2C) receptor gene polymorphisms and smoking initiation in male Caucasian subjects.

• 出版日期2010-4-1