Methylation analysis of multiple genes in blood DNA of Alzheimer%26apos;s disease and healthy individuals

作者：Tannorella Pierpaola; Stoccoro Andrea; Tognoni Gloria; Petrozzi Lucia; Salluzzo Maria Grazia; Ragalmuto Alda; Siciliano Gabriele; Haslberger Alexander; Bosco Paolo; Bonuccelli Ubaldo; Migliore Lucia; Coppede Fabio

来源：Neuroscience Letters, 2015, 600: 143-147.

DOI：10.1016/j.neulet.2015.06.009

• DNA methylation
• Alzheimer's disease
• Folate
• Homocysteine
• Vitamin B12
• PSEN1
• BACE1
• MTHFR
• DNMTs
• APOE

We collected blood DNA from 120 late-onset Alzheimer's disease (AD) patients and 115 healthy matched controls and analysed the methylation levels of genes involved in amyloid-beta peptide production (PSEN1 and BACE1), in DNA methylation (DNMT1, DNMT3A and DNMT3B), and in one-carbon metabolism (MTHFR), searching for correlation with age and gender, with biomarkers of one-carbon metabolism (plasma homocysteine, and serum folate and vitamin B12 levels), and with disease status (being healthy or having AD). We also evaluated the contribution of the APOE epsilon 4 allele, the major late-onset AD genetic risk factor, to the studied gene methylation levels. All the genes showed low mean methylation levels (<5%) in both AD and control DNA, no difference between groups, and no correlation with the studied biomarkers, except for MTHFR that showed methylation levels ranging from 5% to 75%, and correlation with circulating biomarkers of one-carbon metabolism. However, mean MTHFR methylation levels were similar between groups (31.1% in AD and 30.7% in controls, P = 0.58). Overall, present data suggest that none of the studied regions is differently methylated in blood DNA between AD and control subjects.

• 出版日期2015-7-23