Adipose Stromal Vascular Fraction Isolation A Head-to-Head Comparison of 4 Cell Separation Systems #2

作者：Aronowitz Joel A^*; Lockhart Ryan A; Hakakian Cloe S; Birnbaum Zoe E

来源：Annals of Plastic Surgery, 2016, 77(3): 354-362.

DOI：10.1097/SAP.0000000000000831

• SVF
• stromal vascular fraction
• stromal vascular fraction isolation
• ADSC
• adipose-derived stem cells
• StemSource 900/MB
• Cytori
• MediKhan
• LipoKit
• SVF-2
• GID Europe
• MultiStation
• PNC International
• SVF isolation

Introduction: With stromal vascular fraction (SVF) cell and adipose-derived stem cell-based technologies translating into the clinical setting, numerous isolation systems have been developed for the point of care isolation of SVF cells from adipose tissue. A relative lack of performance data on these systems can make objective assessment difficult for prospective clinicians. This study compared the performance of 4 SVF cell isolation systems.
Methods: Four isolation systems were compared: the MultiStation by PNC International, the LipoKit by MediKhan, the GID SVF-2 platform by GID Europe Ltd, and the StemSource 900/MB system by Cytori Therapeutics, Inc. Identical lipoaspirate samples for 5 separate donors were used. Stromal vascular fraction output was compared in terms of nucleated cell yield, viability, residual collagenase activity, sterility of the output, colony-forming unit-fibroblast frequency, frequency of CD31-/CD34+/CD45-cells, and operating statistics.
Results: Mean process time ranged from 65.4 to 120.8 minutes. Mean nucleated cell yield per milliliter of tissue processed ranged from 1.01 x 10(5) cells/mL to 6.24 +/- 10(5) cells/mL. Mean cellular viability ranged from 50.3% to 84.02%. Residual collagenase activity was negligible across all systems. Observed colony-forming unit-fibroblast frequency ranged from 0.495% to 1.704%. No significant difference was observed in frequency of CD31-/CD34+/CD45-cells. Results of the anaerobic/aerobic cultures were mixed.
Conclusions: There was considerable variability between the outputs of each system. The system used by a clinician should be tailored to the individual needs of the practice. There is a range of cost options available. This study may help clinicians make more educated decisions when choosing an isolation system to meet their clinical needs.

• 出版日期2016-9