Protein-Based SERS Technology Monitoring the Chemical Reactivity on an alpha-Synuclein-Mediated Two-Dimensional Array of Gold Nanoparticles

作者：Lee Daekyun; Choe Young Jun; Lee Minwoo; Jeong Dae H; Paik Seung R^*

来源：Langmuir, 2011, 27(21): 12782-12787.

DOI：10.1021/la203124e

The enhancement of weak Raman signals has been challenged to obtain high-quality signals of surface-enhanced Raman scattering (SERS). By employing the Parkinson's disease-related protein of alpha-synuclein, we introduce SERS-active gold nanoparticles (AuNPs) individually isolated with an ultrathin alpha-synuclein shell and their 2-D array into a tightly packed monolayer on a glass support, which permits a quantitative SEAS measurement of phthalocyanine tetrasulfonate (PcTS), a chemical ligand of the pathological protein. Subsequently, the PcTS-bound SERS substrate was also shown to be capable of discriminating two biologically important metal ions of iron and copper by detecting copper ion to the sub-ppm level in a highly selective manner via the in situ chemical reaction of metal chelation to PcTS. The strategy of using the protein-based 2-D AuNP SERS platform, therefore, could be further developed into a custom-made protein-based biosensor system for the detection of not only specific chemical/biological ligands of the immobilized coat proteins but also their biochemical reactivities.

• 出版日期2011-11-1