Alleviative effects of deep-seawater drinking water on hepatic lipid accumulation and oxidation induced by a high-fat diet

作者：Chen I Shu; Chang Yuan Yen; Hsu Chin Lin; Lin Hui Wen; Chang Ming Hsu; Chen Jr Wei; Chen Sheng Shih; Chen Yi Chen^*

来源：Journal of the Chinese Medical Association, 2013, 76(2): 95-101.

DOI：10.1016/j.jcma.2012.10.008

• deep-seawater drinking water
• lipid homeostasis
• liver lipid peroxidation
• nonalcoholic hepatosteatosis

Background: Hepatic steatosis is defined as excessive amounts of triglyceride and other fats inside liver cells and has become an emergent liver disease in developed and developing countries. %26lt;br%26gt;Methods: Deep seawater (DSW)300, DSW900, and DSW1500 drinking waters were formulated via a combination of reverse osmosis and electrodialysis. Hamsters on a high-fat diet were assigned to drink the follbwing solutions: (1) normal distilled water, (2) DSW300, (3) DSW900, or (4) DSW1500. Serum, liver, and fecal biochemical values, expression of hepatic genes related to fatty-acid homeostasis, as well as liver antioxidative levels were measured after a 6-week feeding period. Additionally, hematoxylin and eosin staining was used to investigate the liver histopathology. %26lt;br%26gt;Results: Serum/liver lipids, liver sizes, liver malondialdehyde content, and serum aspartate aminotransferase and alanine aminotransferase of high-fat diet hamsters were reduced (p %26lt; 0.05) by drinking DSW, while daily fecal lipid and bile acid outputs were increased (p %26lt; 0.05). DSW drinking water maintained (p %26lt; 0.05) higher liver glutathione and Trolox equivalent antioxidant capacity levels. Although hepatic sterol regulatory element-binding protein-1c, acetyl-CoA carboxylase, fatty acid synthase, and malic enzyme gene expression were not (p %26gt; 0.05) altered, DSW drinking water upregulated (p %26lt; 0.05) hepatic peroxisome proliferator-activated receptor-alpha, retinoid X receptor alpha, and uncoupling protein-2 gene expression in high-fat diet hamsters. The lipid droplets in livers were also reduced in DSW-drinking-water groups as compared to those only drinking distilled water. %26lt;br%26gt;Conclusion: DSW shows a preventive effect on development of hepatosteatosis induced by a high-fat diet.

• 出版日期2013-2