A microfabricated electrical differential counter for the selective enumeration of CD4+T lymphocytes

作者：Watkins Nicholas N; Sridhar Supriya; Cheng Xuanhong; Chen Grace D; Toner Mehmet; Rodriguez William^*; Bashir Rashid

来源：Lab on a Chip, 2011, 11(8): 1437-1447.

DOI：10.1039/c0lc00556h

We have developed a microfabricated biochip that enumerates CD4+ T lymphocytes from leukocyte populations obtained from human blood samples using electrical impedance sensing and immunoaffinity chromatography. T cell counts are found by obtaining the difference between the number of leukocytes before and after depleting CD4+ T cells with immobilized antibodies in a capture chamber. This differential counting technique has been validated to analyze physiological concentrations of leukocytes with an accuracy of similar to 9 cells per mu L by passivating the capture chamber with bovine serum albumin. In addition, the counter provided T cell counts which correlated closely with an optical control (R-2 = 0.997) for CD4 cell concentrations ranging from approximately 100 to 700 cells per mL. We believe that this approach can be a promising method for bringing quantitative HIV/AIDS diagnostics to resource-poor regions in the world.

• 出版日期2011