A scalable, fully automated process for construction of sequence-ready barcoded libraries for 454

作者：Lennon Niall J; Lintner Robert E; Anderson Scott; Alvarez Pablo; Barry Andrew; Brockman William; Daza Riza; Erlich Rachel L; Giannoukos Georgia; Green Lisa; Hollinger Andrew; Hoover Cindi A; Jaffe David B; Juhn Frank; McCarthy Danielle; Perrin Danielle; Ponchner Karen; Powers Taryn L; Rizzolo Kamran; Robbins Dana; Ryan Elizabeth; Russ Carsten; Sparrow Todd; Stalker John; Steelman Scott; Weiand Michael; Zimmer Andrew; Henn Matthew R; Nu**aum Chad

来源：Genome Biology, 2010, 11(2): R15.

DOI：10.1186/gb-2010-11-2-r15

We present an automated, high throughput library construction process for 454 technology. Sample handling errors and cross-contamination are minimized via end-to-end barcoding of plasticware, along with molecular DNA barcoding of constructs. Automation-friendly magnetic bead-based size selection and cleanup steps have been devised, eliminating major bottlenecks and significant sources of error. Using this methodology, one technician can create 96 sequence-ready 454 libraries in 2 days, a dramatic improvement over the standard method.

• 出版日期2010