A novel human gene spindlin 1, encoding a protein localized in the cell nucleus and inducing NIH3T3 cell';s transformation

作者：Gao YH; Qin LP; Zhang P; Chen L; Yuan HF; Bai CX; Yan F; Yue W^*; Pei XT

来源：Progress in Natural Science, 2004, 14(11): 966-+.

DOI：10.1080/10020070412331344641

• spindlin1
• gene transfection
• subcellular localization
• tumorigenesis

A novel human gene, spindlin 1, recently cloned in our laboratory, is highly expressed in the tissue of ovary cancer. To study its biological function, a vector expressing green fluorescent-spindlin 1 fusion protein was constructed and transfected into COS-7 and NIH3T3 cells by lipofectamine methods. The results showed that the fusion protein pEGFP-N1-spindlin 1 was localized in the nucleus of COS-7 and NIH3T3 cells. NIH3T3 cells which could stably express spindlin 1 as a result of RT-PCR analysis compared with the parental NIH3T3 cells displayed a complete morphological change, improved the cell growth and increased the percentage of cells in G(2)/M phase (12.6% vs control cells at 3.4%). Furthermore, overexpressed spindlin 1 cells formed colonies in soft agar, more motile in migration assay in vitro and formed tumors in nude mice. Our findings provide direct evidence that spindlin 1 gene may be a prooncogene which is associated with tumorigenesis.

• 出版日期2004-11