Morphologic and biometric evaluation of chick embryo eyes in ovo using 7 Tesla MRI

作者：Lindner Tobias^*; Klose Ronja; Streckenbach Felix; Stahnke Thomas; Hadlich Stefan; Kuehn Jens Peter; Guthoff Rudolf F; Wree Andreas; Neumann Anne Marie; Frank Marcus; Glass Aenne; Langner Soenke; Stachs Oliver

来源：Scientific Reports, 2017, 7(1): 2647.

DOI：10.1038/s41598-017-02755-4

The purposes of this study were (1) to characterize embryonic eye development during incubation in ovo and (2) to analyze the putative influence of repetitive ultrahigh-field MRI (UHF-MRI) measurements on this development. A population of 38 fertilized chicken eggs was divided into two sub-groups: two eggs (Group A) were examined repeatedly during the developmental period from embryonic day 1 (E1) to embryonic day 20 (E20) to evaluate the influence of daily MRI scanning. A second larger group of 36 eggs was examined pairwise on one day only, from E3 to E20, and the embryos were sacrificed immediately after MR imaging (Group B). Fast T2-weighted MR sequences provided biometric data on the eye with an in-plane resolution of 74 mu m. The data show rapid growth of the eye with a steep increase in intraocular dimensions in all axis directions and in eyeball volume during initial development up to E10, followed by a phase of reduced growth rate in later developmental stages. Comparison of the two groups revealed no differences in ocular development.

• 出版日期2017-6-1