Ang II induce kidney damage by recruiting inflammatory cells and up regulates PPAR gamma and Renin 1 gene: effect of beta carotene on chronic renal damage

作者：Kaliappan Gopal; Nagarajan P; Moorthy Ramya; Selvi S Kalai Gana; Raj T Avinash; Kumar Jerald Mahesh^*

来源：Journal of Thrombosis and Thrombolysis, 2013, 36(3): 277-285.

DOI：10.1007/s11239-012-0835-2

• Chronic renal damage
• apo E-/-mice
• Antioxidant

Antioxidants are widely used for prevention of diseases associated with oxidative stress and ischemic disorder. We investigated the hypothesis of antioxidants (alpha-tocopherol and beta-carotene) can suppress the renal disorder in apo E-/-mice. Renal damage induced by chronic infusion of Angiotensin II (Ang II) into 4 month old male apo E-/-mice. After that the mice were treated with diet enriched alpha tocopherol and beta carotene (800 mg/kg) for 150 days. Ang II treated kidney showed polycystic appearance with accumulation of clear fluid and constriction of renal artery and renal vein was noticed. Vacuolar/cystic degeneration as well as inflammatory reactions was noticed in the tubules/glomerulus of Ang II treated mice. beta carotene treated mice showed enormous numbers of regenerated tubules in the kidney and over expression of ICAM proteins in the regenerated tubules. CD 45.2, MAC 3 proteins were over expressed in the inflammatory cells infiltrated into the tubular region of Ang II treated kidney. Gene expression studies revealed up regulation of Renin 1 (Ren 1) and PPAR gamma genes in the kidney of Ang II treated animals, but the beta carotene treatment controlled the expression of these genes in the regenerated kidneys. beta carotene may have protective effective on chronic renal disorder. It may repress the inflammatory genes (Ren 1, PPAR gamma) to achieve the protective effect on Ang II induced renal damage.

• 出版日期2013-10