A heptameric peptide isolated from the marine microalga Pavlova lutheri suppresses PMA-induced secretion of matrix metalloproteinase-9 through the inactivation of the JNK, p38, and NF-kappa B pathways in human fibrosarcoma cells

作者：Ko, Seok-Chun; Heo, Seong-Yeong; Choi, Sung-Wook; Qian, Zhong-Ji; Heo, Soo-Jin; Kang, Do-Hyung; Kim, Namwon; Jung, Won-Kyo^*

来源：Journal of Applied Phycology, 2018, 30(4): 2367-2378.

DOI：10.1007/s10811-018-1443-0

• Marine microalga
• Pavlova lutheri
• Matrix metalloproteinase-9 (MMP-9)
• Nuclear factor-kappa B (NF-kappa B)
• Mitogen-activated protein kinases (MAPKs)

In this study we isolate a heptameric peptide from the marine microalga Pavlova lutheri and examine its inhibitory effect and mechanism of action on the matrix metalloproteinase-9 (MMP-9) protein in human fibrosarcoma HT1080 cells. Through consecutive rounds of purification we isolated a potent MMP-9 inhibitory peptide composed of seven amino acids: Leu-Leu-Ala-Pro-Pro-Glu-Arg (796.4 Da). We show that the mRNA and protein expression levels of MMP-9 are reduced in the presence of this purified peptide. Furthermore, we show that this peptide inhibits PMA-induced translocation of nuclear factor-kappa B (NF-kappa B) by suppressing I kappa B-alpha phosphorylation and that it suppresses the phosphorylation of the c-Jun N-terminal (JNK) and p38 mitogen-activated protein kinases (MAPKs). Our results indicate that this peptide, purified from P. lutheri, has the potential to be a valuable natural preventive agent against MMP-related diseases.

• 出版日期2018-8
• 单位广东海洋大学