The role of protein sulfhydryl groups and protein disulfides of the platelet surface in aggregation processes involving thiol exchange reactions

作者：Margaritis Antonios; Priora Raffaella; Frosali Simona; Di Giuseppe Danila; Summa Domenico; Coppo Lucia; Di Stefano Anna; Di Simplicio Paolo^*

来源：Pharmacological Research, 2011, 63(1): 77-84.

DOI：10.1016/j.phrs.2010.09.004

• Platelets
• Protein sulfhydryl groups (PSH)
• Protein disulfides (PSSP)
• Plasma thiols
• Integrin alpha IIb beta 3
• Thiol/disulfide exchange reactions

Blood platelets are central to haemostasis and platelet aggregation is considered to be a direct index of platelet function. Although protein disulfides (PSSP) are structural components of most proteins, current evidence suggests that PSSP work together with protein SH groups (PSH) to activate various platelet functions in dynamic processes involving thiol/disulfide exchange reactions.
Based on these assumptions, we performed experiments to demonstrate how PSH and PSSP are involved in platelet aggregation and how modifications of PSH and PSSP concentrations on the platelet surface by N-ethylmaleimide (NEM) (a PSH-blocking reagent) and dithiothreitol (DTT) (a PSSP-reducing reagent), respectively, may condition platelet susceptibility in protein rich plasma and washed platelets and integrin alpha IIb beta 3 conformation. Our data strongly suggest that the PSH blockage and the PSSP reduction of the platelet surface are deeply involved in aggregation processes evoked in protein rich plasma and washed platelets by ADP and collagen; that endogenous thiols (e.g. GSH) may interfere with NEM actions: that NEM and OTT, acting on preexisting PSH and PSSP of active platelets have opposite conformational changes on integrin alpha IIb beta 3 conformation. Although the precise mechanism and the populations of specific PSH and PSSP involved remain unresolved, our data support the notion that PSH and PSSP of the platelet surface are involved in platelet activation by thiol exchange reactions. A plausible molecular mechanism of PSH and PSSP recruitment during thiol exchange reactions is here proposed.

• 出版日期2011-1