Anti-tumour effects by a trimodal combination of temozolomide, meloxicam and X-rays in cultures of human glioma cells

作者：Van Nifterik Krista A^*; Van Den Berg Jaap; Slotman Ben J; Van Rijn Johannes

来源：International Journal of Radiation Biology, 2011, 87(2): 192-201.

DOI：10.3109/09553002.2010.519423

• trimodal treatment
• temozolomide
• meloxicam
• X-rays
• glioma cells

Materials and methods: aEuro integral Cells were exposed to TMZ (96 hours) and MLC was co-incubated during the last 24 h. Thereafter, cells were irradiated with X-rays and plated for a clonogenic assay. Total cell numbers and the numbers of surviving cells were determined to study the recovery of the cell populations (up until 19 days) following different combinations of TMZ, MLC and X-rays.
Results: aEuro integral The combination of MLC and TMZ caused an enhanced cytotoxic effect in D384 and Hs 683. Various treatment combinations demonstrated significant radiation enhancement in all three cell lines. Long-term observations of D384 cells demonstrated that the repopulation rates of the surviving cells are far less affected by the various treatment protocols than those from the non-surviving cells.
Conclusions: aEuro integral The present study demonstrates that a combination of TMZ and MLC resulted in a significant potentiation of their cytotoxicity in D384 and Hs683. The combination of these two drugs can also cause considerable enhancement of the radiation response in human glioma cell lines, although only D384 cells benefit from trimodal over bimodal treatment.

• 出版日期2011-2