Amyloid beta-Mediated Zn2+ Influx into Dentate Granule Cells Transiently Induces a Short-Term Cognitive Deficit

作者：Takeda Atsushi^*; Nakamura Masatoshi; Fujii Hiroaki; Uematsu Chihiro; Minamino Tatsuya; Adlard Paul A; Bush Ashley I; Tamano Haruna

来源：PLos One, 2014, 9(12): e115923.

DOI：10.1371/journal.pone.0115923

We examined an idea that short-term cognition is transiently affected by a state of confusion in Zn2+ transport system due to a local increase in amyloid-beta (A beta) concentration. A single injection of A beta (25 pmol) into the dentate gyrus affected dentate gyrus long-term potentiation (LTP) 1 h after the injection, but not 4 h after the injection. Simultaneously, 1-h memory of object recognition was affected when the training was performed 1 h after the injection, but not 4 h after the injection. A beta-mediated impairments of LTP and memory were rescued in the presence of zinc chelators, suggesting that Zn2+ is involved in A beta action. When A beta was injected into the dentate gyrus, intracellular Zn2+ levels were increased only in the injected area in the dentate gyrus, suggesting that A beta induces the influx of Zn2+ into cells in the injected area. When A beta was added to hippocampal slices, A beta did not increase intracellular Zn2+ levels in the dentate granule cell layer in ACSF without Zn2+, but in ACSF containing Zn2+. The increase in intracellular Zn2+ levels was inhibited in the presence of CaEDTA, an extracellular zinc chelator, but not in the presence of CNQX, an AMPA receptor antagonist. The present study indicates that A beta-mediated Zn2+ influx into dentate granule cells, which may occur without AMPA receptor activation, transiently induces a short-term cognitive deficit. Extracellular Zn2+ may play a key role for transiently A beta-induced cognition deficits.

• 出版日期2014-12-23