Analysis and Identification of Geranium by Two-Dimensional Correlation Infrared Spectroscopy

作者：Sun Ren shuang; Jin Zhe xiong; Zhang Zhe peng; Xu Chang hua; Sun Su qin^*

来源：Spectroscopy and Spectral Analysis, 2013, 33(1): 81-84.

DOI：10.3964/j.issn.1000-0593(2013)01-0081-04

• Geranium
• Infrared spectroscopy
• Second derivative spectra
• Two-dimensional Infrared spectroscopy

Tr-step infrared spectroscopy (Fourier transform infrared spectroscopy (FM) combined with second derivative spectra and two-dimensional correlation infrared spectroscopy (2D-COS)) was employed to identify and analyze the main components of Heilongjiang (HUG), Jilin (JLG), Liaoning (LNG) genuine Herba Geranium. The emergence of several characteristic absorption peaks of tannins including 1730 and 1337 cm(-1) and peaks around 1618 and 1318 cm(-1) belonging to calcium oxalate suggested that Herba Geranii contained tannins and calcium oxalate. Differences near 1370 and 1230 cm(-1) were found among the three Herba Geranii. In light of second derivative spectra, four more peaks of tannin components around 1509, 1204, 764 and 763 cm(-1) and evident differences around C=O stretching bands (1750 similar to 1600 cm(-1)) were observed. By 2D-COS spectra with further improved resolution, the three genuine Geraniums were visually distinguished due to their significant differences in auto-peak profile. HUG has 7 auto peaks with a strongest peak around 1621 cm(-1), while JLG and LNG both have only 4 auto peaks with a strongest peak around 1580 and 1659 cm(-1), respectively. It was demonstrated that the Tr-step infrared spectroscopy was successfully applied to fast analyze and identify genuine Geraniums from different geographical regions and subsequently would be applicable to the study of Chinese medicinal resources and quality standards.

• 出版日期2013-1
• 单位清华大学; 哈尔滨商业大学