Modified polysaccharides as potential F-19 magnetic resonance contrast agents

作者：Krawczyk Tomasz^*; Minoshima Masafumi; Sugihara Fuminori; Kikuchi Kazuya

来源：Carbohydrate Research, 2016, 428: 72-78.

DOI：10.1016/j.carres.2016.04.017

• F-19 MRI
• Alginic acid
• Dextran
• Polygalacturonic acid
• HeLa

The introduction of 3-aminobenzotrifluoride into partially oxidized alginic acid, dextran, and polygalacturonic acid (10-100 kDa) by means of the imine formation and a subsequent reduction resulted in water-soluble materials containing 1-14% of fluorine. They showed a single or split F-19 NMR signal in a narrow range of -63 to -63.5 ppm. The observed T-1 and T-2 were approximately 1 and 0.2 s at 400 or 500 MHz instruments, respectively. The samples showed low toxicity and uptake toward the HeLa cells similar to native polysaccharides and were preferentially localized in lysosomes. A tail intravenous injection of 5 mg of modified dextran containing 1% of fluorine revealed that the probe was not trapped in liver, spleen or kidneys, but was quickly cleared with urine. The proposed materials can be used for imaging of the gastrointestinal tract or the genitourinary system and act as a material for more complex F-19 MRI agent synthesis.

• 出版日期2016-6-16