Artemisinin as a Chinese medicine, selectively induces apoptosis in pancreatic tumor cell line

作者：Noori Shokoofe^*; Hassan Zuhair M; Farsam Vida

来源：Chinese Journal of Integrative Medicine, 2014, 20(8): 618-623.

DOI：10.1007/s11655-013-1454-2

• Artemisinin
• endoperoxide bridge
• apoptosis
• reactive oxygen species

To investigate the possible mechanism through which Artemisinin induced apoptosis in pancreatic cell line. %26lt;br%26gt;Column chromatography, thin layer chromatography (TLC) and proton NMR spectroscopy were used to purify Artemisinin. The flowcytometry was employed to detect apoptosis and reactive oxygen species (ROS). %26lt;br%26gt;The results indicated that 50% inhibiting concentration (IC50 value) for pancreatic cell line (RIN) was 45 mu mol/L of Artemisinin. Artemisinin had no cytotoxic effect on the growth of peripheral blood lymphocytes. The mechanism of apoptosis was evaluated by measuring intracellular ROS. It was shown that Artemisinin-induced apoptosis occurred independently of the binding of CD95L to CD95 receptor in the RIN cells. Moreover, Artemisinin, in a dose-dependent manner, could significantly increase the level of ROS. %26lt;br%26gt;Artemisinin can induce apoptosis in the RIN cells via the generation of ROS and triggering the intrinsic pathway of cell death.

• 出版日期2014-8