An Aptamer-based Colorimetric Sensor for Streptomycin and Its Application in Food Inspection

作者：Liu Zhongcheng; Zhang Yanfen; Xie Yao; Sun Ying; Bi Kewei; Cui Zhe; Zhao Lijian; Fan Wufang^*

来源：Chemical Research in Chinese Universities, 2017, 33(5): 714-720.

DOI：10.1007/s40242-017-7029-6

• Aptamer
• Streptomycin
• Systematic evolution of ligands by exponential enrichment(SELEX)
• Au nanopartide
• Aptasensor

An aptamer-based colorimetric biosensor was developed, which could be used to detect residual streptomycin from food quickly and cost-effectively. The ssDNA aptamer target for streptomycin was obtained using systemic evolution of ligands by exponential enrichment through affinity chromatography. A total of 19 candidates were obtained after 10 rounds of selection and were grouped into 3 families according to their similarity of sequence and structure. Among them, aptamer Al5 showed the highest affinity for streptomycin tested by fluorescence intensity analysis with a dissociation constant of 6.07 nmol/L. Aptamer A15 also exhibited a higher streptomycin specificity with the lowest detectable limit of 25 nmol/L according to the value of A(620)/A(520) ratio with AuNP-based colorimetric assay. The method was specific and sensitive for the detection of streptomycin from simply-treated milk and honey at 100 and 125 nmol/L, respectively, and is a promising approach to monitor antibiotics or other small molecules analogues in food inspection.

• 出版日期2017-10
• 单位河北大学