科研之友
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摘要
We developed a liquid chromatography-tandem mass spectrometric (LC-MS/MS) method for the determination of a ginseng saponin metabolite, IH901 or compound K (20-O-beta-D-glucopyranosyl-20(S)-protopanaxadiol) in rat plasma. The method involves protein precipitation by acetonitrile and HPLC separation of the sample extracts on a reversed-phase column (X-terra (TM) RP18) with isocratic elution of 20 mM ammonium acetate and acetonitrile (30:70, v/v), at a flow rate of 0.2 mL/min. The MS analysis was performed by electrospray positive ionization mass spectrometry using multiple reaction-monitoring mode. The mass transitions of IH901 and prednisolone (internal standard) were m/z 640 -> 425 and 361 -> 343, respectively. The standard curves for IH901 were linear over the concentration range of 2.0-500 ng/mL. The lower limit of quantification was 2 ng/mL and the limited of detection was 1 ng/mL for IH901. The coefficient of variation of intra- and inter-day assays at five quality control levels ranged from 2.0 to 13.9% and the accuracy varied between 93.2 and 110.5%. This method was used to determine IH901 in plasma samples after the oral administration of a single 30 mg/kg dose on SD rats.
- 出版日期2005
