Analysis of Helicobacter pylori isolates from Chile: occurrence of selective type 1 Lewis b antigen expression in lipopolysaccharide

作者：Altman Eleonora^*; Fernandez Heriberto; Chandan Vandana; Harrison Blair A; Schuster Myra Wilson; Rademacher Laura Otth; Toledo Claudio

来源：Journal of Medical Microbiology, 2008, 57(5): 585-591.

DOI：10.1099/jmm.0.47783-0

Previous studies have shown that the LPS of Helicobacter pylori isolated from North American and European hosts predominantly expresses type 2 Lewis x (Le(x)) and Le(y) epitopes, whilst the LPS from Asian strains has the capacity to express type 1 Le(a) and Le(b) structures. The aim of this study was to evaluate the expression of Le antigens and the cytotoxin-associated antigen (CagA) by H. pylori isolates from Chile. A total of 38 isolates were screened. The expression of Le antigens and CagA was determined by whole-cell indirect ELISA, using commercially available monoclonal anti-Le and polyclonal anti-CagA antibodies. LPS profiles of H. pylori isolates were assessed by gel electrophoresis and Western blotting. Expression of Le(x) and/or Le(y) epitopes was confirmed in 32/38 isolates (84%), whilst 9/38 isolates (24%) expressed type 1 Le(b) blood group determinants, in addition to type 2 Le(x) and Le(y) structures. Six strains (16%) were non-typeable. The majority of H. pylori strains examined were CagA-positive (83.3%).

• 出版日期2008-5
• 单位河北医科大学