Apoptosis of human pancreatic carcinoma cell-1 cells induced by Yin Chen Hao Decoction

作者：Zhou, Hai-Bo^*; Chen, Jing-Ming; Shao, Li-Ming; Chen, Zhi-Gang

来源：World Journal of Gastroenterology, 2015, 21(27): 8352-8357.

DOI：10.3748/wjg.v21.i27.8352

• Apoptosis
• Pancreatic carcinoma
• Yin Chen Hao Decoction

AIM: To evaluate human pancreatic carcinoma cell line (PANC-1) cells apoptosis and Bcl-2 and Bax expression induced by Yin Chen Hao Decoction (YCHD). @@@ METHODS: The cell growth inhibitory rate was determined by MTT assay. Apoptosis of PANC-1 cells before and after treatment with YCHD was determined by TUNEL staining. Expression of the apoptosis-associated genes, Bcl-2 and Bax, was detected by immunohistochemical staining and reverse transcription -PCR. @@@ RESULTS: YCHD inhibited the growth of PANC-1 cells. Following treatment with YCHD for 24-96 h, the apoptotic rate of PANC-1 cells increased with time. In addition, the positive rate of Bcl-2 protein expression decreased in a time-dependent manner, whereas the positive rate of Bax protein expression increased in a time-dependent manner. Following treatment of with YCHD for 24-96h, expression of BAX mRNA increased gradually and BCL-2 mRNA reduced gradually with time. @@@ CONCLUSION: YCHD induces apoptosis of PANC-1 cells mediated in part via up-regulation of BAX and down-regulation of BCL-2.

• 出版日期2015-7-21
• 单位浙江大学