DEVELOPMENT AMPLIFICATION CONCENSUS GENETIC MARKERS IN BETULA LUMINIFERA BASED ON BIRCH EST DATABASE

作者：Lu Yongquan^*; Li Haiying; Chen Xi; Huang Huahong; Tong Zaikang

来源：Wood Research, 2011, 56(2): 169-177.

• Development
• ACGM
• markers
• Betula luminifera
• birch

By designing primers in the conserved coding sequences flanking a polymorphic site, it is possible to exploit a PCR-based marker called amplified consensus genetic marker (ACGM), which is transferable among closed species. The purpose of this paper was to investigate the feasibility of developing ACGMs in Betula luminifera using birch data. Based on bitch EST data, 109 ACGM candidate primers were developed. Two B. luminifera varieties including Lin'an5 and Sichuan4 which represent two different ecotype were used to verify the candidate ACGM markers. By aligning 3 028 EST sequences from birch with the CDS from Abidopsis using blast, 1227 ESTs could be align successfully. Of these 1227 ESTs, 976 (79.5 %) can design primer in flanking split. We successfully obtained 656 e-PCR products from those 976 putative ACGM loci. And 109 candidates ACGM markers were selected and tested, 105 (96.3 %) yielded stable and clear PCR products in birch. Of those 105 markers, 95 (90.5 %) can work well in at least one B. luminifera genotype. The result: showed that ACGM marker could be a good choice for those species species with scarce available sequence data.

• 出版日期2011