Anti-tumor and immunomodulatory activities induced by an alkali-extracted polysaccharide BCAP-1 from Bupleurum chinense via NF-kappa B signaling pathway

作者：Song, Xiangfu; Ren, Ting; Zheng, Zhou; Lu, Tiancheng; Wang, Zhicheng^*; Du, Fengguo^*; Tong, Haibin^*

来源：International Journal of Biological Macromolecules, 2017, 95: 357-362.

DOI：10.1016/j.ijbiomac.2016.10.112

• Polysaccharide
• Bupleurum chinense
• Immunomodulation

Bupleurum chinense is a well-known traditional Chinese medicine. Polysaccharides extracted from medical plants possess multiple healthy benefits. In the present study, an alkali-extracted polysaccharide (BCAP-1) was isolated from Bupleurum chinense, and evaluated its physicochemical features, anti-tumor activities and immunomodulatory effects. BCAP-1 was obtained by alkali-extraction, ethanol precipitation, and fractionation by DEAE-cellulose and Sepharose CL-6B columns. BCAP-1 markedly inhibited Sarcoma 180tumor growth in tumor-bearing mice, and increased the secretion of TNF-alpha in serum. mu assay showed that BCAP-1 had no cytotoxicity against 5-180 tumor cells. BCAP-1 enhanced the secretion of TNF-alpha and NO, and the transcripts of TNF-alpha. and iNOS were increased. Meanwhile, BCAP-1 treatment induced the phosphorylation of p65 and decreased the expression of I kappa B in macrophages. These results suggest that BCAP-1 could activate macrophages through NF-kappa B signaling pathway, and the anti-tumor effects of BCAP-1 can be achieved by its immunostimulating features.

• 出版日期2017-2
• 单位吉林大学; 吉林农业大学; 北华大学