科研之友
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摘要
alpha E-catenin binds the cell-cell adhesion complex of E-cadherin and beta-catenin (beta-cat) and regulates filamentous actin (F-actin) dynamics. In vitro, binding of alpha E-catenin to the E-cadherin-beta-cat complex lowers alpha E-catenin affinity for F-actin, and alpha E-catenin alone can bind F-actin and inhibit Arp2/3 complex-mediated actin polymerization. In cells, to test whether alpha E-catenin regulates actin dynamics independently of the cadherin complex, the cytosolic alpha E-catenin pool was sequestered to mitochondria without affecting overall levels of alpha E-catenin or the cadherin-catenin complex. Sequestering cytosolic alpha E-catenin to mitochondria alters lamellipodia architecture and increases membrane dynamics and cell migration without affecting cell-cell adhesion. In contrast, sequestration of cytosolic alpha E-catenin to the plasma membrane reduces membrane dynamics. These results demonstrate that the cytosolic pool of alpha E-catenin regulates actin dynamics independently of cell-cell adhesion.
- 出版日期2010-4-19
