Analysis of Fish DNA Biomarkers as a Molecular-Level Approach for Ecological Health Assessments in an Urban Stream

作者：Kang Nami; Kang Han il; An Kwang Guk^*

来源：Bulletin of Environmental Contamination and Toxicology, 2014, 93(5): 555-560.

DOI：10.1007/s00128-014-1392-5

• Pseudogobio esocinus
• Zacco platypus
• Danio rerio
• Genotoxicity
• Stream environmental health assessment

This study was performed to determine the ecosystem health of an urban stream using DNA damage biomarkers of sentinel fish species along with chemical analyses of water and sediments in the impacted and reference zones. The results based on single-cell gel electrophoresis showed that the extent of DNA damage was significantly elevated in the polluted zone. The severity of DNA damage in the impacted zone was largely attributed to high levels of heavy metals, such as As, Zn, Pb, and Ni. The DNA damage biomarker in benthic fish was sensitive to habitat change, which suggested that this fish species and biomarker are excellent tools for risk assessment. The acute toxicity tests demonstrated that SCGE was sufficient to reflect the different genotypes and pollution statuses and to propose the possibility of zebrafish erythrocytes as competitive materials.

• 出版日期2014-11