An adjuvant compound that enhances immunogenicity at fractional doses of the Sabin-inactivated poliovirus vaccine (sIPV) with a long duration of protection in a rat model

作者：Song, Shaohui; Liu, Ze; Zhou, Jian; Cai, Wei; Yang, Huijuan; Ma, Lei; Gao, Jingxia; Li, Weidong^*; Liao, Guoyang^*

来源：Journal of Medical Virology, 2019, 91(1): 14-21.

DOI：10.1002/jmv.25298

• adjuvant
• geometric mean titer
• long duration
• polio vaccine
• seroconversion

Background At the same dosage, the new generation of Sabin-inactivated poliovirus vaccine (sIPV) is less immunogenic than the traditional oral polio vaccine (OPV) dosage in China. The useful adjuvant might be a necessary strategy to strengthen the immune protective effects. Methods Results In this study, we produced an adjuvant compound (named KML05) that could promote immunogenicity and fractional doses of sIPV with a long duration of protection in a rat model. The compound adjuvant had both advantages and a function of MF59 and carbopol971P. The effect seroconversion of experimental animals immunized with KML05 could be extended to one-eighth of the dose. According to the result of the geometric mean titers (GMTs), KML05 adjuvant could save eight times the amount of sIPV D-antigen usage, but aluminum hydroxide adjuvant could save twice at the same titers. Additionally, it was found that there was a significant difference in the GMT titer of poliovirus type 2 between animals immunized by KML05 and alum adjuvant (P < 0.05). At 12th-month postvaccination, the neutralization titers stimulated by IPV-KML05 were maintained over a longer time period in immunized animals. Conclusion Our research team developed KML05 adjuvant, which combined carbopol971P with MF59, increased antibody responses to sIPV for a longer duration of protection in a rat model.

• 出版日期2019-1
• 单位中国医学科学院医学生物学研究所; 中国医学科学院北京协和医院