Adenovirus-mediated expression of spermidine/spermine N1-acetyltransferase gene induces S-phase arrest in human colorectal cancer cells

作者：Sun, Hui; Liu, Bin; Wang, Wei; Jiang, Guang-Shui; Li, Wei; Yang, Ya-Pei; Xu, Chun-Xiao; Yan, Yun-Fei; Liu, Xian-Xi^*

来源：Oncology Reports, 2008, 20(5): 1229-1235.

DOI：10.3892/or_00000134

• spermidine/spermine N-1-acetyltransferase
• colorectal cancer
• polyamine
• cyclin A

Spermidine/spermine N-1-acetyltransferase (SSAT) is a key enzyme of polyamine catabolism. In a previous study, we constructed a recombinant adenovirus, Ad-SSAT, which can express human SSAT. In the present study, we investigated the effect of Ad-SSAT on the growth and cell cycle of colorectal cancer cells. We found that Ad-SSAT increased the expression of SSAT and inhibited the growth of HT-29 and LoVo cells. The growth inhibition was caused by cell cycle arrest in the S phase. Furthermore, Ad-SSAT was shown to suppress the expression of cyclin A and nuclear factor E2F-1 in HT-29 and LoVo cells. The inhibitory effect of Ad-SSAT on cyclin A promoter activity was also observed in a reporter gene assay. Our results suggest that the expression of SSAT mediated by Ad-SSAT infection inhibits the growth of colorectal cancer cells and induces cell cycle arrest at the S phase, through a mechanism involving the suppression of cyclin A and E2F-1 expression.

• 出版日期2008-11
• 单位山东大学