Detection of a novel herpesvirus from bats in the Philippines

作者：Sano Kaori; Okazaki Sachiko; Taniguchi Satoshi; Masangkay Joseph S; Puentespina Roberto Jr; Eres Eduardo; Cosico Edison; Quibod Nina; Kondo Taisuke; Shimoda Hiroshi; Hatta Yuuki; Mitomo Shumpei; Oba Mami; Katayama Yukie; Sassa Yukiko; Furuya Tetsuya; Nagai Makoto; Une Yumi; Maeda Ken; Kyuwa Shigeru^*; Yoshikawa Yasuhiro; Akashi Hiroomi; Omatsu Tsutomu; Mizutani Tetsuya

来源：Virus Genes, 2015, 51(1): 136-139.

DOI：10.1007/s11262-015-1197-6

• Bats
• The Philippines
• Herpesvirus
• Next generation sequencing

Bats are natural hosts of many zoonotic viruses. Monitoring bat viruses is important to detect novel bat-borne infectious diseases. In this study, next generation sequencing techniques and conventional PCR were used to analyze intestine, lung, and blood clot samples collected from wild bats captured at three locations in Davao region, in the Philippines in 2012. Different viral genes belonging to the Retroviridae and Herpesviridae families were identified using next generation sequencing. The existence of herpesvirus in the samples was confirmed by PCR using herpesvirus consensus primers. The nucleotide sequences of the resulting PCR amplicons were 166-bp. Further phylogenetic analysis identified that the virus from which this nucleotide sequence was obtained belonged to the Gammaherpesvirinae subfamily. PCR using primers specific to the nucleotide sequence obtained revealed that the infection rate among the captured bats was 30 %. In this study, we present the partial genome of a novel gammaherpesvirus detected from wild bats. Our observations also indicate that this herpesvirus may be widely distributed in bat populations in Davao region.

• 出版日期2015-8