Metabolic engineering of Corynebacterium glutamicum for the production of 3-hydroxypropionic acid from glucose and xylose

作者：Chen, Zhen^*; Huang, Jinhai; Wu, Yao; Wu, Wenjun; Zhang, Ye; Liu, Dehua

来源：Metabolic Engineering, 2017, 39: 151-158.

DOI：10.1016/j.ymben.2016.11.009

• 3-Hydroxypropionic acid
• Corynebacterium glutamicum
• Metabolic engineering
• Glycerol pathway
• Lignocellulose

3-Hydroxypropionic acid (3-HP) is a promising platform chemical which can be used for the production of various value-added chemicals. In this study,Corynebacterium glutamicum was metabolically engineered to efficiently produce 3-HP from glucose and xylose via the glycerol pathway. A functional 3-HP synthesis pathway was engineered through a combination of genes involved in glycerol synthesis (fusion of gpd and gpp from Saccharomyces cereuisiae) and 3-HP production (pduCDEGH from Klebsiella pneumoniae and aldehyde dehydrogenases from various resources). High 3-HP yield was achieved by screening of active aldehyde dehydrogenases and by minimizing byproduct synthesis (gapA(A1G) Delta ldhA Delta pta-ackA Delta poxB Delta glpK). Substitution of phosphoenolpyruvate-dependent glucose uptake system (PTS) by inositol permeases (loin) and glucokinase (glk) further increased 3-HP production to 38.6 g/L, with the yield of 0.48 g/g glucose. To broaden its substrate spectrum, the engineered strain was modified to incorporate the pentose transport gene araE and xylose catabolic gene xylAB, allowing for the simultaneous utilization of glucose and xylose. Combination of these genetic manipulations resulted in an engineered C. glutamicum strain capable of producing 62.6 g/L 3 -HP at a yield of 0.51 g/g glucose in fed-batch fermentation. To the best of our knowledge, this is the highest titer and yield of 3-HP from sugar. This is also the first report for the production of 3 -HP from xylose, opening the way toward 3-HP production from abundant lignocellulosic feedstocks.

• 出版日期2017-1
• 单位中国科学院长春应用化学研究所; 清华大学